human bladder carcinoma cell line j82  (ATCC)

Journal: The FASEB Journal

Article Title: Intravesical Delivery of P21 mRNA –Loaded Lipid Nanoparticles as a Tumor Suppressor Replacement Therapy for Bladder Cancer

doi: 10.1096/fj.202600049R

Figure Lengend Snippet: Rationale for selecting CDKN1A /p21 as a therapeutic target in bladder cancer and validation of p21 mRNA expression. (A) Pan‐cancer analysis of CDKN1A alteration frequencies based on TCGA datasets. The analysis was performed using the TIMER3 web server. (B) CDKN1A mRNA expression in bladder cancer and normal bladder tissues, shown as log2(TPM + 1), where TPM denotes transcripts per million. Data were analyzed using the TIMER3 web server. (C) Representative p21 immunohistochemical staining images from a bladder cancer tissue microarray stratified by pathological stage (Tis, T1, T2, T3, and T4). Scale bars, 200 μm. (D) Quantification of p21 H‐scores in Tis, T1, T2, T3, and T4 lesions. Compared with Tis lesions, T1, T2, T3, and T4 tumors showed significantly lower p21 H‐scores. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01 (one‐way ANOVA). (E) Western blot analysis of basal and p21 mRNA‐induced p21 protein expression in T24 and J82 bladder cancer cells. Basal p21 protein levels were very low in both cell lines, whereas transfection of p21 mRNA led to robust p21 expression. For p21 blots, both short‐exposure (se) and long‐exposure (le) images are shown. β‐Actin serves as a loading control. (F) Immunofluorescence analysis of EGFP‐HA and p21‐HA expression in T24 and J82 cells using an anti‐HA antibody. Nuclei were stained with DAPI. The p21‐HA signal was predominantly localized to the nucleus, whereas EGFP showed diffuse cytoplasmic distribution. Scale bars, 20 μm.

Article Snippet: The human bladder carcinoma cell line J82 and the human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (ATCC).

Techniques: Biomarker Discovery, Expressing, Immunohistochemical staining, Staining, Microarray, Western Blot, Transfection, Control, Immunofluorescence

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Effect of curcumin on cell proliferation and mean of IC 50 values of K9TCC-PU AXA ( A ), AXC ( B ), and SH ( C ) canine and human T24 ( D ) cell lines after 72 h of incubation. *** p < 0.001 and **** p < 0.0001. Vertical intermittent lines show IC 50 dose and colored dots represent the drug dose (ng/mL).

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Effect of D6 turmeric on cell proliferation and mean IC 50 values of K9TCC-PU AXA ( A ), AXC ( B ), and SH ( C ) canine and human T24 ( D ) cell lines after 72 h of incubation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, and ns = not significant. Vertical intermittent lines show IC 50 dose and colored dots represent the drug dose (µL/mL).

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Effect of mitoxantrone hydrochloride on cell proliferation and mean IC 50 values of K9TCC-PU AXA ( A ), AXC ( B ), and SH ( C ) canine and human T24 ( D ) cell lines after 24 h of incubation. ** p < 0.01, *** p < 0.001, and **** p < 0.001. Vertical intermittent lines show IC 50 dose and colored dots represent the drug dose (µM).

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Annexin V-FITC/PI after 72 h of incubation with curcumin in canine AXA, AXC, and SH, and human T24 tumor cells. The upper left quadrant (yellow) represents the group of necrotic cells, and the one on the upper right (blue), the group of cells in late apoptosis. The cells in the lower right quadrant (green) represent the group of cells in early apoptosis and the lower left quadrant (pink), the viable cells.

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: The cell migration assay of human urothelial bladder carcinoma cells (T24) treated with curcumin, D6 turmeric, and mitoxantrone hydrochloride and their respective vehicles. The bar graphs represent the mean account of cells in four regions of each well randomly ( left ). The representative microscopy images of the migration assay show T24 cells treated with compounds or vehicles for 24 h, Giemsa, obj 200× ( right ). *** p < 0.001; **** p < 0.0001, and ns = not significant.

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Cell Migration Assay, Microscopy, Migration